十九世纪,罗马尼亚存在着一个人鬼共存的神秘地带――特兰西尼亚古镇。镇上有一支邪恶势力在天黑后统治着该地区,那就是吸血鬼德古拉(理查德o劳斯伯格 Richard Roxburgh 饰)领导下妖魔鬼怪。他们一到日黑就出来大肆杀戮,手段异常残忍。更加恐怖的是,这个队伍在不断壮大中,噬血的科学怪人也不远万里投靠了德古拉。
罗马尼亚的天主教在多次征剿失利后,远赴美国请来了吸血鬼猎人范海辛(休o杰克曼 Hugh Jackman 饰)。范海辛带着助手前往特兰西尼亚途中,巧合下救了另一位吸血鬼女猎人――安娜(凯特o贝金赛尔 Kate Beckinsale 饰)。安娜因为家族世代受到德古拉的诅咒而家破人亡,从小练就了一套猎鬼本领。安娜和范海辛一拍即合,共同前往对付德古拉……
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